摘要
禽流感病毒核蛋白(NP)在病毒的转录、复制以及决定病毒的宿主特异性方面都具有重要作用。通过酵母双杂交系统筛选与核蛋白相互作用的蛋白,为进一步了解NP蛋白与细胞内蛋白质的相互关系以及流感病毒与宿主的相互关系奠定基础。应用酵母双杂交系统,构建NP诱饵质粒,进而筛选人脑cDNA文库,寻找可能与禽流感病毒NP相互作用的蛋白质。经过酵母双杂交共验证,得到7个与NP相互作用的阳性克隆。该结果为深入了解病毒复制的分子机理及其在蛋白质水平上与宿主蛋白的相互作用关系提供了线索。
Avian influenza virus Nucleoprotein （NP） is important in viral transcription, replication and determining host specificity of influenza virus. Yeast two-hybrid technique was applied to screen for proteins interacting with virus nucleoprotein, so as to further elucidate the interaction between virus nucleoprotein and cellular proteins, as well as the interaction between virus and host. To explore new proteins interacted with NP protein, a human brain cDNA library was screened using yeast two-hybrid system with NP as the bait. DNA inserts of the positive AD/library plasmids were sequenced. By the BLAST analysis against the GenBank databases seven positive clones resulted in seven genes. Our results could help for the further study on the molecular mechanism of virus replication, transcription and protein-protein interaction. Further investigations were needed to characterize these interactions.
出处
《生物工程学报》
CAS
CSCD
北大核心
2010年第8期1037-1041,共5页
Chinese Journal of Biotechnology
基金
国家重点基础研究发展计划(973计划)(No.2010CB534000)
转基因生物新品种培育重大专项(No.2009ZX08009-141B)
国家科技重大专项课题(Nos.2008ZX10403
2009ZX10004-109)资助~~
关键词
禽流感病毒核蛋白
人脑cDNA文库
酵母双杂交系统
蛋白间相互作用
Avian influenza virus Nucleoprotein, human brain cDNA library, yeast two-hybrid system, protein-protein interaction
