摘要
以米曲霉沪酿3·042为出发菌株,在适宜条件下制备原生质体,并进行紫外线诱变提高蛋白酶活力。经过原生质体紫外诱变的处理和遗传稳定性实验,最终得到一株高产菌株,其蛋白酶活力的产量达1700U/g以上,约是出发菌株的1·8倍。
Aspergillus oryzae Shanghai brewing 3.042 was used as the initial strain to prepare protoplasts under appropriate conditions,and then the method of ultraviolet mutagenesis was applied to the protoplasts in order to increase protease activity of the initial strain.After UV mutagenesis treatment of protoplasts and genetic stability test,a high protease-producing strain with 1700U/g was obtained,it was 1.8 times than the initial strain.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第9期195-196,200,共3页
Science and Technology of Food Industry
基金
安徽省重大科技攻关项目(8010302088)
安徽省高校自然科学基金资助项目(KJ2009A034)
关键词
米曲霉
原生质体
诱变
蛋白酶
Aspergillus oryzae
protoplasts
mutagenesis
protease
