摘要
随着培养时间的延长，日本血吸虫童虫培养细胞的超微结构发生一系列渐进性变化，主要表现在：核中异染色质含量逐渐增加，由０ｄ时的显著少于常染质转变为５４ｄ时的高于常染色质；线粒体逐渐变性，嵴由清晰变为模糊以至消失，基质电子密度降低，最终成为空泡；内质网由环状变为短管状、囊泡状，最后消失。日本血吸虫童虫培养细胞中线粒体和内质网超微结构的这些变化，可以用来判断培养条件的优劣；而从染色质的一系列变化推断，０～１８ｄ是诱导培养细胞发生增殖的最佳时期。
Ultrastructures of cultured cells from schistosomula Schistosoma japonicum changed progressively along with the prolongation of culture time. Heterochromatin in the nucleus increased, e.g., it was less in the first day and more in the fiftyfourth day than euchromatin. Mitochondria degenerated gradually. Cristae blurred little by little and disappeared after 30 days. Electron density of matrix became lower and lower. At last, mitochondria became vacuolar completely. Endoplasmic reticula changed from circular arrangement into short tubes and vacuoels. They disappeared finally. Presumably, the ultrastructural changes of mitochondria and endoplasmic reticula could be used to evaluate whether the culture conditions were appropriate. And the changes of chromatin indicated that 018 days were the best time for the inducement of cell proliferation.
出处
《中国寄生虫病防治杂志》
CSCD
1999年第2期140-142,共3页
Chinese Journal of Parasitic Disease Control
基金
武汉市青年科技晨光计划
湖北省教委和卫生厅资助
关键词
日本血吸虫
童虫
培养细胞
超微结构
动态变化
Schistosoma japonicum, schistosomula, cultured cells, ultrastructure, dynamic changes
