摘要
目的:研究芍药苷的最佳超临界流体提取条件。方法:根据Box-Behnken的中心组合实验设计原理,在单因素试验的基础上,采用三因素三水平的响应曲面分析法,建立白芍中芍药苷超临界流体提取的二次多项式数学模型,并以芍药苷提取率为响应值作响应面和等高线,考察萃取压力、萃取温度和甲醇浓度对芍药苷超临界提取的影响。结果:芍药苷超临界流体提取的优化工艺条件为:萃取压力为18.9MPa,萃取温度为59.9℃,夹带剂甲醇浓度为68.5%,夹带剂流速0.4mL/min,萃取时间为60min。结论:在此提取条件下,芍药苷的理论提取率可达7.85mg/g。
AIM： To develop a method for optimizing of supercritical fluid extraction （SFE） of paeoniflorin. METHODS： On the basis of one-factor test and the Box-Behnken central-composite design response surface with 3 factors and 3 levels was adopted. A second order quadratic equation for SFE of paeoniflorin was built. Response surface and contour were graphed with the extraction rate of paeoniflorin as the response value. Based on the analysis of the response surface plots and their corresponding contour plots,the effects of extraction pressure,extraction temperature,and methanol concentration were explored. RESULTS： By using the new method discussed above, the optimum extraction condition was obtained as follows： 18. 9 MPa extraction pressure,extraction cosolvent temperature 59. 9 ℃,68. 5% methanol was chosen as cosolvent with the flow rate of 0. 4 mL/min,and extraction time 60 min. CONCLUSION： Under the optimized condition,extraction rate of paeoniflorin on theoretical value is 7. 85 mg/g.
出处
《中成药》
CAS
CSCD
北大核心
2010年第8期1332-1337,共6页
Chinese Traditional Patent Medicine
基金
宁波市教育局资助项目(Jd070204
Jd090222)
关键词
芍药苷
超临界流体提取
响应曲面分析法
优化提取条件
paeoniflorin
supercritical fluid extraction （SFE）
response surface methodology（RSM）
optimization of extraction condition
