摘要
目的：研究、建立中药车前草药材的定性定量方法。方法：以大车前苷（plantamajoside）为专属性指标成分,以硅胶G为吸附剂,醋酸乙酯-甲醇-甲酸-水（18∶3∶1.5∶1）为展开剂,紫外光灯（365 nm）下检测,建立车前草药材的薄层色谱鉴别方法。用高效液相色谱法进行含量测定,艾杰尔Promocil C18色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈-0.1%甲酸水（17∶83）,流速1.0 mL.min-1,检测波长330 nm,柱温30℃。结果：车前草药材薄层色谱在紫外光灯（365 nm）下检识,大车前苷呈亮蓝色荧光斑点,斑点清晰,分离效果理想;高效液相色谱法含量测定大车前苷在0.049 9~11.966 4μg呈线性（r=0.999 9）;加样回收率为100.6%,RSD 2.7%;测得11批车前草中大车前苷的含量在0.067%~1.80%。结论：通过对11批车前草中的大车前苷的定性和定量分析,证明所建立的薄层色谱鉴别和高效液相色谱法含量测定方法灵敏,准确可靠,重复性好,为车前草的品质评价和质量标准建立提供了科学依据。
Plantamajoside is one of the main bioactive compounds in Plantaginis Herba.A TLC method was developed identification of plantamajoside in 11 Plantaginis Herba samples using silica gel G as coating substance and a mixture of ethyl acetiate methanol-formic acid-water（18∶3∶1.5∶1） as a developing solvent,the established TLC condition displayed a very well separation on the chromatogram of tested Plantaginis Herba samples and the marker compound plantamajoside showed as a distinct light-blue fluorescence spot observed under UV 365 nm.Using the HPLC method,plantamajoside was separated at 30 ℃ on a Promocil C18（4.6 mm×250 mm,5 μm） column with acetonitrile-0.1%formic acid（17∶83） as the mobile phase.The detection wavelength was set at 330 nm and the flow rate was 1 mL·min-1.The calibration curve of plantamajoside displayed ideal linearity over the range of 0.049 9-11.966 4 μg（r=0.999 9）,and the average recovery of plantamajoside was 100.6%with a RSD of 2.7%.The contents of plantamajoside were in the range of 0.067%-1.80% in Plantaginis Herba.The established TLC identification and HPLC were sensitive,reliable and repeatable,which can be applied for the quality evaluation and standard criteria of Plantaginis Herba.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第16期2095-2098,共4页
China Journal of Chinese Materia Medica
基金
国家药典委员会《中国药典》2010版一部标准研究课题(YD-017)
国家“重大新药创制”重大专项(2009ZX09502-013,2009ZX09502-024)
上海科委中药现代化专项基金(08DZ1970103)
