摘要
目的阐明ＨＢＶ对感染细胞凋亡的影响。方法ＨｅｐＧ２及其转染ＨＢＶ的ＨｅｐＧ２２１５细胞培养中，加ＭＴＸ、ＡｃｔＤ或去血清培养，以ＦＣＭ检测细胞凋亡率。结果ＨｅｐＧ２．２．１５细胞加ＭＴＸ后２４小时和４８小时，凋亡率分别为１０８％和１３３％，加ＡｃｔＤ后分别为１６８％和３７７％，去血清后第４天及第６天，分别为１３２％和１４８％；而ＨｅｐＧ２细胞加ＭＴＸ后２４小时和４８小时，凋亡率则为１２６％和６５３％，加ＡｃｔＤ后分别为４４５％和８９７％，去血清后第４天和第６天凋亡率为１９８％和２８８％。结论在上述条件下，ＨｅｐＧ２２１５细胞较ＨｅｐＧ２细胞耐受凋亡；ＨＢＶ可能抑制肝癌细胞凋亡。
Objective To elucidate the effect of HBV on hepatocyte apoptosis. Methods The HepG2 cells and the HBV transfected HepG2.2.15 cells were cultured with MIX or ActD or cultured in DMEM medium without serum, The apoptosis was examined with FCM. Results The apoptotic rates of HepG2.2.15 cells at 24 h and 48 h after MTX addition were 10.8% and 13.3%, at 24 h and 48 h after Act D addition were 16.8% and 37.7%, and at 4 th and 6 th day after serum withdrawal were 13.2% and 14.8%, respectively. While those of HepG2 cells were 12.6% and 65.3%, 44.5% and 89.7%, and 19.8% and 28.8%, correspondingly. Conclusion HepG2.2.15 cell was tolerant to these apoptotic stimuli, and it might be inferred that HBV inhibits hepatocyte apoptosis.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1999年第2期142-144,共3页
Chinese Journal of Experimental and Clinical Virology
