摘要
目的建立适于临床检测原发上皮性卵巢癌ＭＤＲ１ｍＲＮＡ表达方法。方法应用半定量ＲＴ－ＰＣＲ技术对３７例原发上皮性卵巢癌、１０例正常卵巢和２０例外周血新鲜标本ＭＤＲ１基因进行了检测。结果原发上皮性卵巢癌标本中ＭＤＲ１基因阳性表达率为３０％，而正常卵巢和外周血标本则无ＭＤＲ１基因表达。结论ＲＴ－ＰＣＲ技术检测原发上皮性卵巢癌ＭＤＲ１基因表达具有灵敏度高、相对定量和结果可靠的优点，适于临床应用。
Objective To establish a method for measuring expression of MDRl gene in primary epithelial ovarian carcinoma (PEOC). Methods A sensitive semiquantitative RT-PCR technique was used for detection of MDRl gene expression in 37 PEOC ,10 normal ovarian epithelial tissues and 20 normal femal human peripheral blood monuclear cells (PBMC). Results MDR1 gene expression was detected in 30% of tumors analysed,but there was no expression in normal ovarian epithelial tissues and PBMC. Conclusion RT-PCR assay is highly sensitive ,relatively quantitative and reliable for measuring levels of MDRl gene expression in clinical samples.
出处
《济宁医学院学报》
1999年第2期4-6,共3页
Journal of Jining Medical University
关键词
卵巢肿瘤
抗药性
基因表达
RT-PCR
Ovarian neoplasm,Multidrug gene expression
RT-Polymerase chain reaction
