摘要
目的：比较伯氏疟原虫氯喹抗性（ＲＣ）株和敏感（Ｎ）株对宿主肝脏细胞色素Ｐ４５０ⅡＢ１基因ｍＲＮＡ转录影响的差异。方法：以小鼠ＣＹＰ４５０ⅡＢ１基因的ｃＤＮＡ（５５２ｂｐ）为探针，分别与感染伯氏疟原虫ＲＣ株和Ｎ株的小鼠肝脏总ＲＮＡ进行Ｎｏｒｔｈｅｒｎｂｌｏｔ杂交。结果：感染伯氏疟原虫Ｎ株小鼠肝ＣＹＰ４５０ⅡＢ１基因的ｍＲＮＡ明显低于感染ＲＣ株鼠及正常对照鼠，而感染ＲＣ株鼠与正常对照鼠之间该基因的转录无明显差异。结论：伯氏疟原虫ＲＣ株和Ｎ株感染对宿主肝脏ＣＹＰ４５０ⅡＢ１基因ｍＲＮＡ转录的影响有显著差异。
Objective: To compare difference of CYP450B1 mRNA transcription in mice liver between Plasmodium berghei chloroquineresistant(RC)and chloroquinesensitive (N) strains. Methods: Total RNA extracted from livers infected with the RC and N strains was assayed by Northern blot using cDNA (552 bp) of CYP450B1 gene as probe. Results: The transcription of CYP450B1 gene in livers infected with the N strain was evidently lower than that of infected with the RC strain and normal control.However,there was no significant difference between mice infected with the RC strain and normal control. Conclusion: There is a difference between the N and RC strains in the CYP450B1 gene transcription of their hosts. It is demonstrated that at molecular level that chloroquineresistance mechanism may speed up the drug metabolism in their hosts.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1999年第6期365-367,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
