摘要
通过正交实验法,研究愈创木酚与复合碳源共降解对黄孢原毛平革菌产酶的影响,同时探讨不同分子结构对酶催化机制的影响。实验结果表明:同时添加愈创木酚及不同结构的碳源对该菌产酶有显著影响,高浓度的愈创木酚对产酶有明显的促进作用;在培养基中添加愈创木酚2 mmol/L、葡萄糖2.5 g/L和糊精5 g/L,可以显著提高黄孢原毛平革菌的综合产酶能力;黄孢原毛平革菌可以分泌纤维素酶和木聚糖酶,而且二者之间有较好的线性正相关关系。
The influence of guaiacol and compounded carbons sources co-degradation on enzymes of white-rot fungi was studied through the orthogonal experiment.The results show that carbons with different structures and guaiacol have remarkable effects on enzymes secreting by Phanerochaete chrysosporium.High concentration of guaiacol can enhance enzymes production.After optimumization of various factors,the addition of guaiacol 2 mmol/L,glucose 2.5 g/L,dextrine 5 g/L in culture medium can significantly promote enzymes production.CMCase and xylanase produced by Phanerochaete chrysosporium are little affected by exterior environment.According to the correlativity between the CMCase and the xylanase analyzed using linear regression,a positive correlation the CMCase and the xylanase is found.
出处
《中南大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2010年第4期1628-1632,共5页
Journal of Central South University:Science and Technology
基金
国家重点基础研究发展规划("973"计划)项目(2005CB724203)
国家杰出青年科学基金资助项目(50425927)
国家教育部长江学者和创新团队发展计划项目(IRT0719)
国家自然科学基金青年科学基金资助项目(50808073)
湖南省环境保护科技项目(2007185)
关键词
愈创木酚
复合碳源
黄孢原毛平革菌
guaiacol
compounded carbons
Phanerochaete chrysosporium
