摘要
目的：探讨宫内缺氧后胎鼠肾内血管内皮生长因子（VEGF）、环氧化酶2（COX-2）的表达及意义.方法：孕21d健康SD大鼠,制作胎鼠宫内缺氧模型,取胎鼠肾组织H-E染色,VEGF、 COX-2免疫组织化学显色. 结果：缺氧组新生鼠VEGF、 COX-2阳性细胞数和积分光密度值均随缺氧时间延长而增大. 结论：一定时间宫内缺氧可刺激胚胎大鼠肾内VEGF和COX-2的表达增加,COX-2与VEGF表达呈明显的正相关.
Objective： To study the expression of vascular endothelial growth factor （VEGF） and cyclooxygenase-2 （COX-2） in the kidneys of fetal rats following intrauterin hypoxia. Methods： 21 d-conceiving healthy SD rats were used to establish the intrauterine hypoxia model of fetal rats, and then their kidney tissues were taken out immediately after intrauterin hypoxia being stained by H E. VEGF and COX-2 expression were detected by immunohistochemistry. Results： The number of VEGF and COX-2 positive cells and the IOD of VEGF and COX-2 positive cells in the hypoxia group was increased with hypoxia time increasing. Conclusion ： Hypoxia can increase the expression of VEGF and COX-2 in the kidneys. The expression of COX-2 is positively correlated with that of VEGF.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2010年第4期472-474,483,共4页
Chinese Journal of Anatomy
关键词
缺氧
肾
血管内皮细胞生长因子
环氧化酶2
hypoxia
kidney
vascular endothelial growth factor
cyclooxygenase-2
