摘要
采用稀释分离法从辽宁沈阳市土壤中分离到1株放线菌Snea49。对Snea49进行了形态学特征、培养特征、生理生化特征及16S rDNA分析,初步鉴定为环状链霉菌(Streptomyces anulatus)。利用离体测试法,研究了Snea49菌株代谢物不同倍数稀释液对大豆胞囊线虫的抑制作用。结果表明:10倍稀释液浓度下,该菌株对胞囊孵化的相对抑制率达82.60%,与无菌水对照差异显著。处理24 h后,在1倍稀释液浓度下,该菌株对2龄幼虫的校正死亡率是89.66%,各稀释浓度均与无菌水对照差异显著,该菌株有较高的杀线活性。
Strain Snea49,was isolated by diluted solution from a soil sample collected in Liaoning province.The results based on characteristics of morphological,physiology and biochemistry and 16S rDNA sequence analysis of strain Snea49 showed that it was Streptomyces anulatus.Fermentation filtrates of Streptomyces anulatus at different concentrations were evaluated for nematicidal activity against soybean cyst nematode in the laboratory,bioassays included egg hatching and mortality of second stage juvenile.The hatching rate of cysts were suppressed by 82.60% at 10× diluted solution.The corrected mortality rates of juveniles were 89.66% when J2 were treated with the strain at 1× dilution for 24 hours.The difference between these treatments and water control were significant.Fermentation filtrates of the strains were effective in inhibiting H.glycines hatch and killing J2.
出处
《大豆科学》
CAS
CSCD
北大核心
2010年第4期663-665,共3页
Soybean Science
基金
教育部新世纪优秀人才支持计划资助项目(NCET-08-0866)
辽宁省优秀人才支持计划资助项目(RC-05-18)
