摘要
目的观察金雀异黄素(Gen)对高级蛋白氧化产物(AOPPs)刺激下SD大鼠肾小球系膜细胞(MC)分泌TGF-β1的影响。方法以体外培养的SD大鼠MC为研究对象,分为对照组、AOPPs组和Gen组。检测细胞培养液中TGF-β1的浓度,并明确量效关系。结果AOPPs刺激的MC与对照组比较,细胞培养液中TGF-β1的浓度升高,而Gen能抑制上述效应,且具有时间和浓度依赖性。结论在体外培养条件下,Gen能抑制AOPPs刺激下MCTGF-β1表达的增加,且作用具有时间和浓度依赖性,其机制可能均与Gen灭活氧化应激有关,提示Gen对AOPPs刺激下的MC具有保护作用。
Objective To investigate the effect of genistein（Gen）on expression of TGF-β 1secreting by cultured rat MC stimulated by advanced oxidation protein products（AOPPs）.Methods The MC rats were divided into three groups as control group,AOPPs group and Gen group.The quantity of TGF-β 1was determined by ELISA in vitro.Results The content of TGF-β1 increased in MC of AOPPs group more significantly than that of control group.Gen could inhibit the effect of AOPPs with dose and time dependence.Conclusion AOPPs could promote the expression of TGF-β1 in MC in vitro.Gen could down-regulae the secreting of TGF-β1 with time and dose dependence.The mechanisms might have something to do with the antioxidant effect of Gen.
出处
《实用药物与临床》
CAS
2010年第4期244-245,共2页
Practical Pharmacy and Clinical Remedies
