摘要
目的:通过正交实验,确定火棘果红色素的最佳提取工艺。方法:以贵州野生火棘果为原料,首先,以柠檬酸的乙醇溶液为提取剂,在400～800nm范围内对提取液的吸收光谱进行扫描,确定其最大吸收波长;然后,以最大吸收波长处的吸光度为衡量指标,确定最佳提取剂;再次,探讨了提取次数、提取温度、提取时间、料液比等单因素对火棘果红色素提取效果的影响;最后,在单因素实验结果的基础上,通过L9(34)正交实验,确定红色素的最佳提取工艺。结果:1.在400～800nm范围内,火棘果红色素的最大吸收波长为530nm;2.最佳提取剂为9%柠檬酸乙醇溶液;3.火棘果红色素的最佳提取工艺为,料液比1∶10,提取时间60min,提取次数2次,提取温度40℃。
Objective：According to the orthogonal experimental design the optimum extracting conditions. of Red Pigment from Pyracantha fortuneana Fruit. Methods ： Taking natural Pyracantha fortuneana fruit as raw material, in order to determine the maximum absorption wavelength, the extraction of red pigment was scand under UV - Vis spectrophotometer in the 400 - 800 nm range of the absorption spectra. Then, according to the size of absorbance in the maximum absorption wavelength to determine the best extraction agent; third, the effection of single factor on the extraction rate were investgated, such as the number of extraction, extraction temperature, extraction time, material ratio. Finally,based on the single factor experiment, the optimum extracting conditions were determined through orthogonal experiment L9 （34）. Resuits ： between 400- 800 nm, the longest absorptional wavelength was 530nm; the best extractor was eth- anol mixed with 9% citric acid; the optimum extracting conditions of red pigment from pyracanthafortuneana fruit were as followes： the material ratio of 1：10. ,time of 60 minutes, extraction times of 2 times and temperature of 40%.
出处
《中国野生植物资源》
2010年第4期50-53,共4页
Chinese Wild Plant Resources
关键词
紫外-可见分光光度方法
火棘
红色素
提取
UV - VIS Spectrophotometer
Pyracantha fortuneana
red pigment
extraction
