摘要
本文先用计算机预测、后用51Cr4h释放分析证实的方法，对丙型肝炎病毒（HCV）核心区蛋白（C蛋白）上，HLA－A2限制性CTL识别表位进行了鉴别。结果发现，2名感染HCV的HLA－A2阳性供血员的外周血单个核细胞（PBMc）对ALAHGVRAL（core150～158）肽段标记的自体靶细胞有溶解作用，杀伤率分别为37．5％和15．8％；用抗CD4mAb阻断后杀伤率无明显变化，而用抗CDSmAb阻断后明显减少，提示该条肽段可能为HLA－A2限制性CTL识别表位。
In this Paper, we attempted to idenop epitopes recognized by the HLA-A2 metricted cytotobo T lymphocyte (CTL)on hepatihs C virus (HCV) core protein utilizing the methed of computer prwhction before conforming by 4 h 31Cr-release assay. The results showed that Peripheral bled mononuclear cells(PBMCS) Obtained fro two HLA-A2 posihve donors who infected with HCV could lyse autolognus target cells theied vitb peptide 'AraHGVRAL(core 150 ～ 158)'. The specific lysis of the cells from the two donors were 37. 5% and 15. 8%, mepechvely. Blocking of the CTL response with and-CD4 showed that the specific lysis had no signifcant decrease. But blocking of CTL resPOnse with and-CDS could abolish the lysis. h indicated that the peptide was the Candidate epitope recwind by the HLA-A2 restricted CTL.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1999年第1期10-13,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金!39570634
