摘要
建立离子色谱法同时分离检测蜂蜜和葡萄酒中的蔗糖、葡萄糖和果糖的分析方法。用METROSEPCARB1(150mm×4.0mm)阴离子交换分离柱和脉冲安培检测器,对淋洗分离条件进行优化。选择10mmol/LNaOH为淋洗液,淋洗液流速1.0mL/min,总分析时间25min。蔗糖、葡萄糖、果糖的检测限分别为0.085、0.126、1.072mg/L(进样体积20μL)。混标溶液连续8次进样,峰高的相对标准偏差为3.12%～10.56%,保留时间的相对标准偏差为2.36%～3.25%。样品测定的回收率为90.0%～99.5%。该方法具有样品前处理简单,灵敏度高,快速有效等优点,可为蜂蜜和葡萄酒质量检测提供一个简单有效的方法。
An ion chromatographic method has been developed for the determination of sucrose, glucose and fructose in honey and wine. The optimal chromatographic conditions were investigated for the separation on METROSEP CARB 1 （150 mm × 4.0 mm） anion exchange column. Pulsed amperometric detector was used for detection. Elutions were performed using 10.0 mmol/L NaOH at a flow rate of 1.0 mL/min. The total analysis time was 25 min. The detection limits for sucrose, glucose, fructose were 0.085, 0.126 mg/L and 1.072 mg/L （20 μL injection）, respectively. The RSDs of peak height for 8 consecutive determinations were between 3.12% and 10.56%, and the RSDs of retention time were between 2.36% and 3.25%. The spike recoveries ranged from 90.0% to 99.5%. This method is characteristic of simple sample pretreatment, high sensitivity and shorter analysis time, and can provide a simple and efficient method for the determination of honey and wine.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第16期188-191,共4页
Food Science
基金
重庆市自然科学基金项目(CSTC2007BB5370)
国家科技部重大专项项目(2008ZX07315)
关键词
离子色谱
脉冲安培检测
蔗糖
葡萄糖
果糖
ion chromatography
pulsed amperometric detection
sucrose
glucose
fructose
