摘要
总RNA的提取是进行实时荧光定量PCR,Nothern杂交分析c,DNA文库的建立等分子生物学研究的基础。概述了目前国内外细菌总RNA提取方法的现状,对提取过程中的常见问题进行了分析,并且对其未来的发展趋势进行了展望。
Extraction of total RNA is the basis of real-time fluorescence quantitative PCR,Nothern hybridization analysis,cDNA library creation and other molecular biology research.The status quo of extraction methods for bacterial total RNA in China and abroad was summarized;the common problems in the extraction process were analyzed and its development prospects were brief introduced.
出处
《湖南农业科学》
2010年第3期9-11,共3页
Hunan Agricultural Sciences
基金
国家863计划重点项目(2008AA100804)
上海市科委重大科技攻关项目(07dz19508)
"十一五"国家科技支撑计划课题专项(2009BAK43B17)
