摘要
目的:筛选出卷叶贝母鳞茎再生的最佳离体培养条件。方法:通过对卷叶贝母开花期鳞茎的离体培养,研究了不同光照条件、培养温度、激素配比以及病毒唑用量对卷叶贝母鳞茎再生的影响。结果与结论:卷叶贝母鳞茎外植体进行适当的低温暗处理后,再转入光下培养,可明显地减轻外植体的褐化程度;鳞茎再生的最适培养温度为(20±2)℃,激素对鳞茎再生有明显的促进作用,其中以6-BA 2.0 mg/L和NAA 0.2 mg/L组合为最佳;浓度为10 mg/L的病毒唑最适于鳞茎的再生。
Objective： The optimal in vitro culture conditions for the regeneration of Fritillaria cirrhosa were screened.Methods： Through the in vitro culture of the flowering stage Fritillaria cirrhosa,the effect of illumination,culture temperature,hormone combination and the amount of ribavirin on the regeneration of Fritillaria cirrhosa were studied.Results and Conclusion： The explant browning level could be apparently reduced if the bulb were cultured under the ray after exposure to proper low-temperature.The optimal temperature for the regeneration of bulb is（20 ± 2） ℃.The hormone has obvious promotional effect on the regeneration of bulb,and the best combination is 6-BA 2.0 mg/L plus NAA 0.2 mg/L.Concentration of 10 mg/L of ribavirin is best for the regeneration of bulb.
出处
《中药材》
CAS
CSCD
北大核心
2010年第6期854-856,共3页
Journal of Chinese Medicinal Materials
基金
国家"十一五"科技支撑计划重点项目(2006BAI06A13)
四川省科技支撑计划(2010SZ0007)
关键词
离体培养条件
卷叶贝母
鳞茎再生
In virtor culture conditions
Fritillaria cirrhosa D.Don
Bulb regeneration
