摘要
目的:了解2009年兰州地区5岁以下婴幼儿轮状病毒(Rotavirus,RV)腹泻流行状况及毒株变异特点,为疫苗的研发提供流行病学资料。方法:在哨点监测医院(兰州大学第一医院)收集5岁以下住院患儿粪便标本313份,采用ELISA检测RV,阳性标本用RT-PCR进行G、P分型。结果:2009年RV检出率为50.80%(159/313),腹泻高发季节在10～12月。G分型中以G3型(51.57%)为主,其次是G1(7.55%)、G2(6.29%)和G9(5.66%)。P分型中以P[8]型(24.14%)为主。G-P组合主要以P[8]G3型(33.82%)、P[4]G3型(19.12%)、P[4]G2型(5.88%)和P[8]G1型(1.47%)为主,另外还发现P[6]G3型、P[6]G9型、P[4]G9型、P[8]G9型等较少见的组合型。结论:RV是引起兰州地区婴幼儿腹泻最主要的病原,毒株呈现型的多样性变化。
Objective：To study the epidemiological status on rotavirus diarrhea among children aged 0～5 years old in Lanzhou in 2009,and provide the epidemic and variation data of rotavirus strain for the development of rotavirus vaccine.Methods：313 stool specimens were collected from the First Hospital of Lanzhou University,and were detected by ELISA Kits.Serotype G and genotype P identifications of the rotavirus were determined by RT-PCR.Results：RV was detected in 159 of 313（50.80%） specimens.RV diarrhea admissions peaked between October and December.G3（51.57%） was the predominant strain followed by G1（7.55%）、G2（6.29%） and G9（5.66%）.The most common G-P type combinations were P[8]G3（33.82%）,P[4]G3（19.12%）,P[4]G2（5.88%）,P[8]G1（1.47%）,and other uncommon strains of the type were P[6]G3,P[6]G9,P[4]G9 and P[8]G9.Conclusion：RV was a major pathogen of diarrhea in children aged 0～5 years old in Lanzhou,and the virus variation of the epidemical strains were multiplex.
出处
《中国卫生检验杂志》
CAS
2010年第8期2025-2026,2028,共3页
Chinese Journal of Health Laboratory Technology
关键词
轮状病毒
血清型
基因型
腹泻
监测
Rotavirus
Serotype
Genotype
Diarrhea
Surveillance
