摘要
采用聚丙烯酰胺垂直板电泳技术对小麦族内的加拿大披碱草、野大麦及其杂种Ｆ１和蒙古冰草、航道冰草及其杂种Ｆ１、ＢＣ１的过氧化物酶（ＰＯＤ）同工酶做了比较研究。结果表明，每种植物的ＰＯＤ酶谱可明显分成Ａ、Ｂ两区，在Ｂ区有２条位点相同的酶带，为亲本的基带；所有供试材料呈现６～９条酶带，亲本与其杂种后代的酶谱表型有一定差异；ＰＯＤ同工酶具多态性，可作遗传标记用于杂种鉴定和回交后代目标性状植株的检测；加拿大披碱草×野大麦Ｆ１具双亲各自酶谱的强带，可能Ｆ１具两亲本的某些优良性状。
The POD isozyme of Elymus
canadensis, Hordeum brevisbulatum and their F 1 hybrid, Agropyron mongolicum, Agropyron
cristatum and their F 1 hybrid and backcross 1 in Triticeae were analyzed by polyacrylamide
gel electrophoresis. The results showed: the POD zymograms of each species can be
divided into two regions (A and B). There were two isozyme bands at same location, which
were parental base bands. All experiment materials had 6～9 isozyme bands. There were
differences in the zymograms between parents and their hybrids. There was polymorphism in
the POD isozyme and it can be used as genetic marker to identify hybrid and to determine the
plants with object characteristics in backcross descendants. The E. canadensis-H.
brevisubulatum F 1 hybrid had parental isozyme bands with higher activity. It probably
possesses of some superior characteristics of parents.
出处
《中国草地》
CSCD
1999年第2期4-7,共4页
Grassland of China
基金
国家自然科学基金
