摘要
目的：利用酸灌注的方法制备兔实验性食管炎模型．方法：将胃管插入家兔食管至食管下端括约肌（LES）上方约5cm处，灌注0．1mol／LHCl（1mL／min，30min／d），连续灌注4d，同时于灌注前及灌注后测定食管下端括约肌（LES）的压力．结果：酸灌注后的食管病理改变明显，光镜下可见食管下段粘膜基底细胞层明显增生，固有层中性粒细胞浸润．电子显微镜下可见细胞间隙增加，平滑肌细胞内线粒体肿大，嵴溶解．灌注前后LES的压力分别为（1．400±0．136）kPa和（0．707±0．115）kPa．结论：连续酸灌注的方法可以制备兔实验性食管炎模型，同时使LES的压力明显下降．
AIM: To establish the experimental esophagitismodel in rabbits with acid perfusions. METHODS:By 30 minperfusions of 0. 1 mol/L HCl 5 cm above the LES for fourconsecutive days,the model was established. The LES pressure was measured before and after each perfusion. RESULTS: Significant inflammatory changes were observed under the light microscope and electronic microscope after theacid perfusions, LES pressure is (1. 400± 0. 136) kPa beforeand (0. 707 ±0. 115 ) kPa after the perfusions. CONCLUSION: Using acid perfusions for four consecutive days, experimental esophagitis model could be established in rabbits,and LES pressure drops significantly.
出处
《第四军医大学学报》
1999年第2期152-154,共3页
Journal of the Fourth Military Medical University
