摘要
目的：为了查明引起2008-2009年浙江省疑似病毒性脑炎的病原,分析病原的分子特征。方法：采用Hep-2和RD细胞从疑似患者脑脊液样本中分离肠道病毒,对分离株扩增VP1基因,测序并进行同源性与进化分析。结果：从患者11份脑脊液样本中分离到4株柯萨奇B1病毒（CVB1）;测得VP1基因全长为834核苷酸（nt）,无插入和缺失,推导编码278个氨基酸（aa）。4株浙江CVB1分离株（ZJ/CVB1）之间在VP1区nt和aa的同源性分别为96.3%~99.9%和100%。浙江CVB1分离株BLAST比对nt同源性最高的毒株为2006年山东株（SD/168/06）,同源性为98.7%;与CVB1原型株（Conn-5/86）的nt同源性为78.9%。VP1区氨基酸进化树显示浙江CVB1分离株在CVB1 Clade3进化支上,与浙江株亲缘关系最近的毒株为山东CVB1株,均在同一进化支上。结论：从病毒分离结果证实浙江省疑似病毒性脑炎是由肠道病毒CVB1引起,2008-2009年在浙江循环的CVB1病毒之间没有明显差异,浙江CVB1流行株与山东株亲缘关系最近。
Objective：To confirmed the cause of the aseptic meningitis and clarified the molecular characteristic of pathogen in Zhejiang during 2008-2009.Methods：The virus were isolated from cerebrospinal fluid（CSF） samples of the suspected cases by Hep-2 and RD cell lines.The RNAs of the isolated viruses were extracted and followed by RT-PCR to amplify VP1 gene,which was then sequenced,and the phylogenetic and homology trees were also constructed.Results：4 out of 11 CSF samples were positive of isolated of Coxsackievirus B1（CVB1）.The complete VP1 gene of CVB1 virus strains from Zhejiang（ZJ/CVB1） was 834 nt without any insert or deletion,which encoded 278 amino acid（aa）.The identity of VP1 gene in nt and aa among 4 sequenced were separately 96.3%～99.9% and 100%.The Shandong strain（SD/168/06） had the highest identity of VP1 gene which the ZJ/CVB1 compared with the BLAST program,the identity was 98.7%.The identity of VP1 gene of ZJ/CVB1 and prototype strain（Cnno-5/86）was 78.9%.The phylogenetic tree indicated that ZJ/CVB1 had maximum similarity with SD/168/06.They all lied in the CVB1 Clade 3.Conclusion：The virus isolation identified that the pathogen of the aseptic meningitis in Zhejiang was caused by enterovirus CVB1.There was no obviously difference among ZJ/CVB1 strains in 2008-2009.There was the most genetic relationship between ZJ/CVB1 and SD/168/06.
出处
《中国卫生检验杂志》
CAS
2010年第9期2134-2136,共3页
Chinese Journal of Health Laboratory Technology
关键词
病毒性脑炎
柯萨奇B1
VP1基因
分子特征
Aseptic meningitis
Coxsackievirus B1
VP1 gene
Molecular characteristic
