摘要
无花果曲霉是一种可以产植酸酶的菌株，其代谢产物植酸酶可以将有机植酸磷降解为无机磷。以此菌株为出发菌株，确定了它的最适pH值为1．3—1．4，最适温度为55-60℃。同时，为获得高酶活的突变株，进行亚硝基胍和紫外线处理，经初筛得到99株高效突变株，再经复筛和传代试验，得到1株植酸酶活性是出发菌株2．47倍的突变株NTG-23。
Aspergillus ficuum is a strain that could produce phytase. Its metabolites could degrade organic phosphate into inorganic phosphate. It was taken as a starting strain to begin with the study. And confirmed pH 1.3 ~ 1.4 was the most suitable value and optimum temperature was 55 -60 ℃ respectively. In order to obtain mutant strain with high enzymatic activity, UV and NTG treatment were taken and obtained 99 mutant strains with highly effective through primary screening. One strain （NTG-23） was obtained through secondary screening and generation passing experiment, its enzymatic activity was 2.47 times of the starting strain.
出处
《微生物学杂志》
CAS
CSCD
2010年第4期83-87,共5页
Journal of Microbiology
基金
国家"十一五"科技支撑计划项目(2006BAD12B05)
动物营养学国家重点实验室自主研究课题(2004DA125184G0811)
