摘要
目的:研究乳腺癌组织中死亡相关蛋白激酶(death-associated protein kinase 1,DAPK1)启动子甲基化状态及其与临床病理特征之间的关系,并探讨该甲基化状态与DAPK1 mRNA表达的相关性。方法:应用甲基化特异性PCR法检测人乳腺癌组织和相应癌旁正常乳腺组织中DAPK1启动子甲基化状态,分析其与患者临床病理特征的关系,并结合半定量RT-PCR法的检测结果加以分析。结果:43例乳腺癌标本中DAPK1启动子甲基化阳性率为44.1%(20/43),DAPK1启动子甲基化状态与年龄、肿瘤大小、组织学分级、TNM分期、ER状态、PR状态、Her2状态无关(P>0.05),而与有无淋巴结转移、P53是否阳性有关(P<0.05)。DAPK1启动子甲基化标本中的DAPK1mRNA表达水平低于未甲基化标本,两者差异具有统计学意义(P<0.05)。结论:乳腺癌中DAPK1基因启动子区高甲基化与其mRNA失表达有关,在乳腺癌发生、发展中可能起重要作用,有可能作为乳腺癌诊断和预后分析的检测指标之一。
Objective To explore the relationship between the methylation of death-associated protein kinase 1（DAPK1） promoter and the clinical pathological characters in breast cancer and the correlation between the methylation of DAPK1 promoter and DAPK1 mRNA expression.Methods Methylation-specific PCR was performed to detect the methylation status of the DAPK1 promoter in human breast tissue.The results were analyzed by combination with the clinical and pathological data of the patients and the RT-PCR results.Results Among the 43 breast cancer samples,DAPK1 promoter methylation was detected in 20 cases（44.1%）.The methylation of DAPK1 promoter was not correlated with age,TNM stages,tumor size,estrogen receptor status,progesterone receptor status or human epidermal growth factor receptor status（P〉0.05）,but it was correlated with lymph node metastasis or P53 status（P〈0.05）.The expression of DAPK1 mRNA was lower in the samples with DAPK1 promoter methylation than that in the unmethylated samples（P0.05）.Conclusion Hypermethylation of the DAPK1 promoter is correlated with the loss of its transcription in breast cancer.The high permethylation of the DAPK1 promoter plays a crucial role in the genesis and development of breast cancer and may be used as one of the biomarkers for diagnosis and prognosis for breast cancer.
出处
《国际病理科学与临床杂志》
CAS
2010年第4期277-281,共5页
Journal of International Pathology and Clinical Medicine
关键词
乳腺癌
死亡相关蛋白激酶1
甲基化
breast neoplasms
death-associated protein kinase 1
methylation
