摘要
目的:研究糖皮质激素(Glucocorticoids,GCS)及卡介苗(Bacille calmette-guerin,BCG)疫苗在治疗分泌性中耳炎(Secretory otitis media,SOM)中的疗效及其作用机制。方法:将24只BALB/C小鼠分为4组,每组6只,其中卵清白蛋白(Ovalbumin,OVA)模型组(OVA组)、卡介苗治疗组(BCG组)、糖皮质激素治疗组(GCS组)腹腔注射卵清白蛋白致敏,然后用卵清白蛋白耳内激发2次;阴性对照组(CON组)腹腔注射生理盐水,然后生理盐水激发2次。BCG组在耳内激发前3d给予双下肢外侧皮内注射卡介苗0.02mg,GCS组在耳内激发前3d给予经口吸入强的松1mg,其它2组不作干预,4组分别于2次激发后2d处死小鼠,中耳组织做病理切片观察其形态学改变,免疫组织化学染色观察IL-4、IL-5、INF-γ、IL-12细胞因子的表达差异情况。结果:BCG组、GCS组中耳粘膜组织炎症反应较OVA组明显减轻,且GCS组与BCG组比较,炎症反应亦明显减轻,Th1型细胞因子IL-12、IFN-γ较OVA组显著增多,(P<0.05);而Th2型细胞因子IL-4、IL-5较OVA组明显减少(P<0.05),BCG在一定程度上扭转了Ⅰ型变态反应的发生发展,而GCS使Th1、Th2、型细胞因子更趋于平衡。结论:卡介苗是有效的免疫调节剂,可能通过调节Th1/Th2的细胞的平衡,达到治疗的作用。而糖皮质激素仍然是变应性炎症疾病治疗中最有效的一类药物,为临床治疗分泌性中耳炎提供依据。
Objective：To study the the mechanismof effect of glucocorticoids and BCGvaccine in the treatment of secretory otitis media. Methods：24 BALB/C mice were divided into 4 groups of 6,The OVA、BCG and GCS groups were immunized with OVA/alum and challenged with OVA.BCG group was injected with BCG 0.02 mg intraperitoneal injection 3 days before being challenged,GCS group was given the oral inhalation prednisone 1 mg for 3 days before being stimulated.And the other two groups have non-intervention,2 days later the mice were killed all,We analysed the pathology observation of the middle ear mucosa,the expression of cytokines such as IL-4、IL-5、 IFN-γand IL-12 by immunohistochemistry.Results：BCG Group,GCS group of middle ear mucosa inflammatory response than the OVA group was significantly reduced,and the GCS group,compared with BCG Group,also significantly reduce the inflammatory.Th1-type cytokines IL-12,IFN-γwas significantly increased than the OVA group,（P0.05）;while Th2-type cytokines IL-4,IL-5 was significantly reduced,（P0.05）.Conclusion：BCG is an effective immunomodulator,probably by regulating the Th1/Th2 balance.And glucocorticoids are still the the most effective drugs in the treatment of allergic inflammatory disease.The study provides the basis for the clinical treatment of secretory otitis media.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2010年第8期1191-1193,共3页
Journal of Chongqing Medical University
