摘要
目的探讨致敏的树突状细胞联合化疗治疗胃肠道恶性肿瘤的IFN-γ、IL-10和IL-12的动态变化,建立评价树突状细胞疗效的特异性免疫指标及毒副作用。方法 40例晚期的胃癌和结直肠癌患者随机分为树突状细胞联合化疗组和单纯化疗组,治疗组化疗前分离患者外周血单核细胞,体外培养成致敏的树突状细胞,培养期间给予患者1周期的5-氟尿嘧啶(5-FU)、亚叶酸钙(CF)和奥沙利铂(L-OHP)方案的化疗,化疗后回输树突状细胞,对照组给予患者同一方案的化疗,治疗前后检测血清中的IFN-γ、IL-10、和IL-12,并观察卡氏评分和毒副作用。结果观察组生物化疗后IFN-γ、IL-12和卡氏评分升高,IL-10下降,对照组化疗后IL-10水平上升,IFN-γ、IL-12和卡氏评分下降,均有统计学意义;观察组行树突状细胞回输时,未发现Ⅲ度以上的毒副作用。结论 IFN-γ、IL-10、IL-12是树突状细胞治疗肿瘤的特异性免疫效果的指标,树突状细胞能够减轻化疗的毒副反应,提高免疫功能,改善患者的生活质量。
To establish the specific immunological indexes of sensitized dendritic cell,we observed the change of IFN-γ,IL10,and IL-12 in the peripheral blood of gastrointestinal cancer patients before and after dendritic cell combined with chemotherapy,and investigated the side effect of the combined chemotherapy in gastrointestinal cancer.Firstly,forty patients with advanced gastric cancer or colorectal cancer were randomly divided into chemotherapy group （control group） and dendritic cell combined with chemotherapy group （observing group）.Then sensitized matured DCs were collected from peripheral blood monocytes of the patients in observing group before chemotherapy,which were loaded tumor lysate and cultivated in vitro.In the course of cultivation,the patients in observing group received Oxaliplatin （L-OHP） plus 5-FU/CF.After chemotherapy,the sensitized matured DC were administered to the patients in observing group,while patients in control group received the treatment of the same regimen except the DC administration.The concentrations of IFN-γ,IL-10,IL-12 in blood serum were detected before and after treatment,at the same time the toxicity and KPS of these treatment were observed.In observing group,the concentrations of IFN-γ and IL-12 in blood serum and the KPS were significantly increased,but the concentration of IL-10 was decreased after treatment;on the other hand,in control group,the concentrations of IFN-γ and IL-12 in blood serum and the KPS were decreased,but the concentration of IL-10 was increased after chemotherapy.In observing group,when sensitized matured DCs were administered,no serious side effect was observed.The results showed that IFN-γ,IL-10,IL-12 are the specific immunological indexes of sensitized dendritic cell therapy,furthermore,dendritic cell can decrease side effect of chemotherapy,enhance immune function,and improve patients＇quality of life.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2010年第8期702-705,709,共5页
Immunological Journal
