摘要
为进一步阐明茉莉酸甲酯(MeJA)诱导植物抗虫的机理,研究外源MeJA对烟草多酚氧化酶(PPO)、过氧化物酶(POD)、脂氧合酶(LOX)活性及编码氢过氧化物裂解酶(HPL)和蛋白酶抑制剂(PI-I和PI-II)基因表达的诱导作用,并对棉铃虫取食烟草叶片面积及其体重进行生物学测定。结果表明,用100μmol/LMeJA处理后,烟草体内PPO、POD和LOX活性显著升高,HPL、PI-I和PI-II表达明显增强,叶片被取食面积减少,棉铃虫体重增加变慢。说明MeJA对诱导烟草抵御棉铃虫有重要作用。
To further clarify the mechanism of plant resistance to insects induced by methyl jasmonate （MeJA）, the inducement of exogenous MeJA on activities of polyphenol oxidase （PPO）, peroxidase （POD）, lipoxygenase （LOX）, and expression of genes encoding hydroperoxide lyase （HPL） and proteinase inhibitor （PI-I and PI-II） in tobacco leaves were studied. And the damaged area of leaves and weight of cotton bollworms were bioassayed. The results showed that after exogenous application of MeJA at a concentration of 100 μmol/L, the activities of PPO, POD and LOX increased significantly. Also, expression ofHPL ,PI-I andPI-II enhanced evidently. The damaged area of tobacco leaves treated with MeJA decreased apparently compared with that of control. The larvae after 72 h feeding on control plants were larger than that on plants treated with MeJA. Together, our findings indicated that MeJA plays critical roles in induced resistance to cotton bollworms in tobacco.
出处
《中国农学通报》
CSCD
北大核心
2010年第17期277-280,共4页
Chinese Agricultural Science Bulletin
基金
天津农学院博士科研启动基金"外源施用茉莉酸和水杨酸对水稻耐盐性影响的研究"(2008D023)
国家质检总局项目"农药及其代谢物在食品加工过程中的残留动态研究"(2008IK151)
关键词
茉莉酸甲酯
烟草
防御酶
防御基因
棉铃虫
诱导抗性
methyl jasmonate
tobacco
defense enzymes
defense genes
cotton bollworms
induced resistance
