摘要
【目的】对一株新分离的、产生柚苷酶的疑似酵母菌株Jmudeb008进行鉴定,确定其分类的种属关系,阐明葡萄糖对该菌株表达柚苷酶的影响。【方法】利用形态观察、核酸分析及生理生化实验对Jmudeb008进行种属鉴定;用含有0.5g/L柚皮苷及不同浓度葡萄糖的培养基培养Jmudeb008,通过检测培养过程柚皮苷、柚皮素及葡萄糖浓度的变化研究葡萄糖对Jmudeb008表达柚苷酶的影响。【结果】Jmudeb008的菌落形态及个体形态都与典型的酵母相似,其26SrDNAD1/D2区域和5.8SrDNA-ITS区域的序列与罗伦隐球酵母（Cryptococcus laurentii）的同源性为99%,葡萄糖发酵试验阴性,尿酶试验阳性,重氮基蓝B试验阳性、硝酸盐还原试验阴性符合该菌种特性,因此鉴定为罗伦隐球酵母。Jmudeb008在以柚皮苷为唯一碳源的培养基中或当培养基中葡萄糖消耗完以后会分泌柚苷酶,而有葡萄糖存在时不分泌柚苷酶。【结论】分离得到了能产柚苷酶的罗伦隐球酵母,该酵母产柚苷酶受葡萄糖分解代谢调节。
[Objective]We identified a new isolated naringinase-producing yeast strain named as Jmudeb008,and analyzed its naringinase-producing ability cultured with different composition and concentration of carbon sources.[Methods]The strain was identified based on conventional phenotypic methods and sequences of the D1/D2 region of 26S rDNA and 5.8S-ITS.Media with different composition and concentration of carbon sources were used in shaking culture of Jmudeb008.The activity of naringinase was evaluated by analyzing the concentration of naringin,naringenin and glucose during 48 h culture.[Results]The Jmudeb008＇s sequences of the D1/D2 region of 26S rDNA and 5.8S-ITS were 99% identical with Cryptococcus laurentii.Further glucose fermentation test,urease test,DBB（diazotization based blue）test and nitrate reduction test were coincided with results of DNA sequencing.Therefore,Jmudeb008 was identified as Cryptococcus laurentii.When Jmudeb008 was cultured in the medium with naringin as the only carbon source,it could synthesize naringinase.However,when glucose was available,the synthesis of naringinase was repressed.[Conclusion]The new isolated naringinase-producing yeast strain Jmudeb008 was identified as Cryptococcus laurentii.The glucose in medium repressed naringinase expression.
出处
《微生物学报》
CAS
CSCD
北大核心
2010年第9期1202-1207,共6页
Acta Microbiologica Sinica
基金
福建省科技计划重点项目(2009N0044)
科技人员服务企业行动项目(2009GJC40050)
集美大学中青年创新团队专项基金(2008A002)
厦门市科技项目(3502Z20093023)~~
关键词
柚苷酶
罗伦隐球酵母
鉴定
葡萄糖
naringinase
Cryptococcus laurentii
identification
regulation of naringinase producing
