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线粒体DNA 5178C/A多态性与2型糖尿病关系的研究 被引量：1

Study on the association of the mitochondrial DNA 5178C/A polymorphism with type 2 diabetes mellitus
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摘要 目的研究线粒体DNA5178C/A多态性与2型糖尿病(T2DM)及其并发症的关系。方法采用聚合酶链反应(PCR)与PCR产物直接测序的方法,对2型糖尿组(220例)及正常对照组(126例)进行基因分析。结果 5178C基因型与5178A基因型的T2DM患者在性别、发病年龄、体重指数、血糖、血脂等水平差异无统计学意义,但T2DM患者中5178C基因型并发视网膜病变的频率要高于5178A基因型(P<0.05)。结论线粒体DNA5178C/A多态性可能与T2DM患者并发视网膜病变负相关。 Objective To investigate the association of the mitochondrial DNA 5178C/A polymorphism with type 2 diabetes mellitus（T2DM） and its complications.Methods The gene was amplified by polymerase chain reaction（PCR）,and sequenced with the direct sequencing method to analyze mit ochondrial DNA 5178C/A polymorphism genotypes of 220 T2DM patients and 126 healthy controls.Results The clinical data of age at onset,sex,BMI,and the serum levels of blood glucose,and blood fat had no significant difference in the 5178C and 5178A genotype of T2DM patients.However,the incidence of retinopathy complication in the 5178C genotype of T2DM patients was significantly higher than that in the 5178A genotype（P〈0.05）.Conclusion The mit ochondrial DNA 5178C/A polymorphism may be negative correlation to retinopathy complication of T2DM patients.
作者 胡晓美 吕建新
机构地区 江苏省扬州市第一人民医院核医学科 温州医学院
出处 《检验医学与临床》 CAS 2010年第17期1820-1822,共3页 Laboratory Medicine and Clinic
关键词 2型糖尿病 线粒体DNA 多态性 type 2 diabetes mellitus mitochondrial DNA polymorphism
分类号 R587.1 [医药卫生—内分泌]
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参考文献13

  • 1李永航.线粒体糖尿病概述[J].辽宁实用糖尿病杂志,2004,12(1):3-5. 被引量：3
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二级参考文献12

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检验医学与临床
2010年 第17期

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