摘要
从人淋巴结中分离与纯化HMGN2蛋白,探讨HMGN2分子抗菌作用及其在淋巴结中的亚细胞分布。我们采用高效液相色谱分离纯化,琼脂糖弥散法筛选纯化组分的抗菌活性,对活性组分进行质谱分析测定精确分子量,进行Western-blot检测同源性。结果成功从人淋巴结中分离纯化出的活性蛋白,对致病性大肠杆菌54080有抗菌活性,质谱精确分子质量测定、Western-blot分析证明此活性蛋白即为HMGN2分子,免疫组化实验结果显示在淋巴结组织细胞细胞浆和细胞核均有HMGN2蛋白分布。从人淋巴结组织成功分离纯化出纯度较高、具有生物活性的HMGN2蛋白,可能参与了体内的天然免疫防御作用。
This study was conducted to isolate and purify antimicrobial polypeptides HMGN2（high mobility group nucleosomal-binding domain2） from human lymph node,to detect the antimicrobial activity of HMGN2,and to determine the subcellular location of HMGN2 in human lymph node.The antimicrobial polypeptides were purified by the Reverse Phase HPLC and identified by Tricine-SDS-PAGE.The antimicrobial activity was detected by agar diffusion test.Mass spectrum and Western-blot analysis indicated the individual character of protein.HMGN2 was isolated and purified from human lymph node,and it showed antimicrobial potency against the pathogenic strain E.coli 54080.The immunocytochemistry staining indicated that HMGN2 was present both in human lymph node cells＇ nucleus and cytoplasm.In conclusion,HMGN2 protein is of antimicrobial activity and it is probably involved in the defence of innate immunity in vivo.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2010年第4期842-846,共5页
Journal of Biomedical Engineering
基金
CMB项目资助(98-681)
国家自然科学基金资助项目(30972764)
关键词
人淋巴结
HMGN2
分离纯化
亚细胞定位
Human lymph node
HMGN2
Isolation and Purification
Subcellular localization
