摘要
以竹园采集土样分离出的根际微生物为主要研究对象,采用16s rDNA分子生物学手段和常规生理生化分析方法,对笋用竹正常生长植株及病害植株进行分析研究。有病竹植株的根际微生物可培养菌落数目比正常竹植株的多。特征分析表明:在分离纯化出的45株细菌中,其中的8株细菌初步鉴定为:菌株5-1-4为地衣芽孢杆菌Bacillus licheniformis,菌株5-1-10为苏云金芽孢杆菌Bacillus thuringiensis,4-1-17为枯草芽孢杆菌Bacillus subti-lis,菌株3-1-8为洋葱博克霍尔德菌Burkholderia cepacia,菌株2-1-9为粘金黄菌Chryseobacterium gleum,菌株4-1-14为短小芽孢杆菌Bacillus pumilus,菌株4-1-16为普通变形菌Proteus vulgaris,菌株4-1-6为产气肠杆菌Enterobacter aerogenes。
The rhizosphere microorganisms were separated from the soil samples collected in bamboo gardens,which grew on the normal bamboos and the sick ones,and were studied by means of the 16s rDNA molecular biology technology and routine analysis of physiology and biochemistry.The number of the culturable bacteria colony from the sick bamboo plants was larger than that from the normal ones.Among the 45 bacteria that were separated and purified out,8 bacteria were identified initially,and they are 5-1-4 Bacillus licheniformis,5-1-10 Bacillus thuringiensis,4-1-17 Bacillus subtilis,3-1-8 Burkholderia cepacia,2-1-9 Chryseobacterium gleum,4-1-14 Bacillus pumilus,4-1-16 Proteus vulgaris,and 4-1-6 Enterobacter aerogenes.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2010年第9期181-186,共6页
Journal of Central South University of Forestry & Technology
基金
中南林业科技大学大学生创新实验项目(2007-1D-GSX)
关键词
植物生理生化
笋用竹
病害植株
根际微生物
plant physiology and biochemistry
shoot-used bamboo species
disease plants
rhizosphere microorganisms
