摘要
目的:检测Survivin和COX-2在人类非小细胞肺癌组织中的表达,探讨二者与非小细胞肺癌(NSCLC)的发生及生物学行为之间的关系。方法:实验组包括非小细胞肺癌60例,对照组包括正常肺组织15例。应用免疫组织化学洁检测Survivin和COX-2的表达水平,并用CD34指示肿瘤微血管计算微血管密度值。结果:非小细胞肺癌患者Survivin和COX-2的表达率分别为38.3%和48.3%,显著高于正常对照组。Survivin和COX-2的表达与临床分期和有无淋巴结转移有关,阳性表达率在临床Ⅲ+Ⅳ期高于Ⅰ+Ⅱ期,有淋巴结转移组高于无淋巴结转移组。Survivin的表达还与组织学分级有关,随着分化程度的降低表达率增加。Survivin和COX-2的表达呈显著正相关关系。非小细胞肺癌组MVD值显著高于正常对照组,并且与临床分期有关,临床Ⅲ+Ⅳ期高于Ⅰ+Ⅱ期。Survivin和COX-2的表达与非小细胞肺癌的微血管密度值(MVD)密切相关S,urvivin和COX-2阳性组MVD值显著高于阴性组。结论:Survivin和COX-2在非小细胞肺癌的发生发展过程中可能起重要作用,其中促进肿瘤血管生成和淋巴结转移可能是影响肿瘤进展的重要途径。
Objective： To detect the expression of survivin and cyclooxygenase-2 （COX-2） in nonsmall cell lung cancer（NSCLC ）, and try to find out associations between their expression and tumorigenesis and development of （NSCLC）. Methods： Sixty cases of （NSCLC）15 cases of normal lung tissue were involved in immunohisochemical staining for survivin and COX-2 expression. CD34 was used to show MVD of samples. Results： Positive rates of survivin and COX-2 expression of （NSCLC） were 38.3%和1 48.3% respectively, which were significantly higher than that of normal control. Survivin and COX-2 expression of III ＋ 1V stage or with lymph node metastasis groups were respectively higher than those of I ＋ II stage or without node metastasis groups. Survivin expression was associated with histologic grade of NSCLC, cases with poor differentiated had higher positive expression rate. There was a positive correlation between expression of Survivin and COX-2. MVD of NSCLC was significantly higher than that of normal control and there was a correlation between MVD and advanced clinic stage. Survivin and COX-2 expression was associated with MVD of NSCLC, cases with survivin and COX-2 positive expression had much higher MVD than survivin and COX-2 negative expression counterpart.Conclusion：Survivin and COX-2 probably play an important role in development of （NSCLC）. Inducing tumor angiogenesis and lymph node metastasis probably are two important ways of Survivin and COX-2 to mediate tumor progression.
出处
《天津医科大学学报》
2010年第3期433-436,共4页
Journal of Tianjin Medical University
