摘要
[目的]探讨三黄汤灌肠对三硝基苯磺酸(TNBS)诱导溃疡性结肠炎(UC)模型大鼠的疗效。[方法]采用TNBS诱导制备UC大鼠模型45只,随机分为模型组、阳性对照组及中药治疗组,各15只,并选未造模大鼠15只作为正常对照组。各组均灌肠给药,模型组和正常对照组予0.9%氯化钠注射液,阳性对照组予柳氮磺吡啶0.5 g/kg,中药治疗组予三黄汤浓煎剂60 g/kg,各组均按10 ml/kg每日灌肠1次。给药时间从造模后第3天开始,连续7 d。第11天观察各组大鼠结肠黏膜损伤指数(CMDI)及黏膜病理组织学情况。[结果]中药治疗组结肠黏膜病理损伤明显改善,CMDI为(1.5±0.535)分,与模型组的(3.55±1.214)分比较差异有统计学意义(P<0.05);与阳性对照组的(2.63±1.188)分比较差异有统计学意义(P<0.05)。在肉眼及电镜下观察发现,中药治疗组大鼠结肠黏膜较模型组、阳性对照组明显改善。[结论]三黄汤灌肠对TNBS诱导的大鼠UC疗效确切,能显著改善结肠黏膜的充血水肿、溃疡、糜烂等。
[Objective]To study the therapeutic effect of Sanhuang decoction on ulcerative colitis（UC）rats induced by trinitrobenzene sulphoacid（TNBS）.[Methods]Forty-five UC rats were induced by TNBS and randomly divided into model group,positive control group and Sanhuang decoction group,with 15 rats in each group.Fifteen normal Wistar rats were set as control group.Model group and normal control group were treated by 0.9% sodium chloride injection,positive control group by Salazosulfapyridine（SASP）（0.5 g/kg）,Sanhuang decoction group by Sanhuang decoction respectively（60 g/kg）,once per day.The treatment was given from the 3rd day after modeling for consecutive 7 days.Changes of CMDI,mucous membrane histopathology were observed on the 11st day.[Results]Compared with model control and positive control groups,CMDI and pathologic injury were markedly improved in Sanhuang decoction group.[Conclusion]Sanhuang decoction is effective in the treatment of UC rats induced by TNBS,ameliorating the congestion,edema,ulcers,erosion of the colonic mucosa.
出处
《中国中西医结合消化杂志》
CAS
2010年第4期220-222,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
