摘要
目的：建立HPLC测定维A酸在小鼠骨髓中含量的方法。方法：色谱柱为Diamonsil C18柱（150mm×4.6 mm,5μm）,流动相为甲醇-水-冰醋酸（95∶5∶0.5）,流速为1.0 mL·min^-1,检测波长为350 nm。外标法定量。结果：维A酸的线性范围为0.01-10μg·mL^-1（r=0.999 9）,最低检测限为0.5 ng。日间与日内精密度均〈5%。低、中、高3个浓度的平均绝对回收率为74.5%,方法回收率为99.3%。结论：该方法灵敏度高、准确,可用于小鼠骨髓组织中维A酸浓度的测定。
Objective： To establish an HPLC method for determining all-trans retinoic acid（ATRA） concentration in mouse bone marrow.Methods： Shimadzu C18（150 mm×4.6 mm,5 μm） was applied;MeOH-H2O-HAc（95∶5∶0.5） was used as a mobile phase;flow rate was 1.0 mL·min^-1;and the detective wavelength was 350 nm.Results： The standard curve of ATRA was linear in the range of 0.01~10 μg·mL^-1（r=0.999 9）.The limit of detection was 〈0.5 ng.The RSDs of intra-day and inter-day were 5%.The average extraction recovery was 74.5%,and the average recovery of the assay was 99.3%.Conclusion： The present study provides a sensitive and accurate method for the determination of ATRA in mouse bone marrow.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2010年第16期1472-1474,共3页
Chinese Journal of New Drugs
关键词
高效液相色谱法
全反式维甲酸
微乳
HPLC
all-trans retinoic acid（ATRA）
micro-emulsion（ME）
