摘要
在原核生物发育分化的分子遗传学研究中，链霉菌因其复杂的分化生命周期，无与伦比的合成次级代谢产物的能力（目前世界上所知的数千种天然抗生素的７０％由链霉菌产生），使之成为原核生物乃至微生物发育与分化研究的最好模式系统［１］，为研究基因时空表达和阐明分化调...
bp of promoter P TH4 was subcloned into Streptomyces plasmid pIJ4083. pIJ4470 was obtained after recombinant plasmid was introduced into Streptomyces coelicolor J1501. Both Streptomyces coelicolor J1501/pIJ4083 and J1501/pIJ4470 were grown on minimal medium (MM) agar containing mannitol and 10μg/mL thiostrepton for 6 days, and then ultrathin sections of colonies were prepaired and stained with silver proteinate to observe glycogen biosynthesis in cell by the electron microscope. Result showed that the glycogen was produced by J1501/pIJ4083, whereas little glycogen was produced by J1501/pIJ4471. The dark color spores were not observed in J1501/pIJ4470 with lead stain in contrast with dark color spores which were produced by J1501/pIJ4083, indicating that promoter P TH4 may play an important role in physilogical and morphological differentiation of Streptomyces.
出处
《微生物学报》
CAS
CSCD
北大核心
1999年第4期373-380,共8页
Acta Microbiologica Sinica
基金
国家自然科学基金
