摘要
目的:改良卷叶贝母现有品种,增加其药用成分含量,丰富遗传育种资源。方法:本研究通过使用低温沙藏层积处理卷叶贝母种子,使其完成生理和形态后熟,再采用不同浓度的秋水仙素溶液对卷叶贝母种子进行不同时间的诱导处理。结果:经植物形态学和染色体数检测,诱导后的材料具有明显的多倍体特征。结论:卷叶贝母种子用30 mg/L的GA3处理32 h,再经沙藏70 d,其种子萌发率为67.0%;沙藏处理成熟后的种子用0.1%的秋水仙素溶液处理48 h,其多倍体诱导率为85.7%。
Objective： In order to improving the existent breeds of Fritillaria cirrhosa,increasing its medicinal ingredients and enriching the resources of heredity breeding.Methods： By using of low-temperature stratification treatment,the seed of Fritillaria cirrhosa completed its after-ripening of physiology and morphology.The induction treatment by different concentrations of colchicine solution and different treatment times for Fritillaria cirrhosa seeds were compared.Result： Detected the plant morphology and chromosome number,it is shown that the induced material obviously possessed the characteristics of polyploid.Conclusion： With the treatment of 30 mg/L GA3 for 32 h and stratification for 70 d,the seed germination rate of Fritillaria cirrhosa reached 67.0%.After treated with 0.1% colchicine solution for 48 h,the stratificationed mature seed showed polyploid inductivity of 85.7%.
出处
《中药材》
CAS
CSCD
北大核心
2010年第8期1201-1204,共4页
Journal of Chinese Medicinal Materials
基金
国家"十一五"科技支撑计划重点项目(2006BAI06A13)
四川省科技支撑计划(2010SZ0007)
关键词
卷叶贝母
种子
萌发
多倍体诱导
Fritillaria cirrhosa D.Don
Seeds
Germination
Polyploid Induction
