摘要
试验建立了猪、牛、绵羊、山羊、鸡、马和牦牛种属鉴别的七重PCR体系,分析了牛、牦牛、山羊源性成分七重PCR检测的灵敏度,检测了10种市售羊肉产品中的动物源性成分。结果表明,采用常规的琼脂糖凝胶电泳可以区分157~517 bp的片段及相互差异在41 bp以上的多重PCR扩增产物,从而实现对这7个物种的快速及准确鉴别,其中对3个物种(牛、牦牛、山羊)DNA的检测灵敏度在2.5 ng左右;所检测的10种羊肉产品中有2种并不是包装上所宣称的羊肉,而是混杂有牛肉或完全用牛肉替代。
Septenary multiplex PCR method and protocol to identify DNAs of pig, cow, sheep, goat, chicken, horse and yak was optimized and developed. Sensitivity of the three species (cow, yak and goat) in the septenary multiplex PCR system was analysed. 10 products made of sheep and goat meat were detected. The results showed that routine agarose gel electrophoresis was sufficient to differentiate the multiplex PCR products ranging from 175 to 517 bp and differing by at least 41 bp each other, indicating that this septenary multiplex PCR method could be used for rapid and accurate identification of the seven species and for three species (cow, yak and goat) at sensitivity of 2.5 ng DNA. Among the 10 products examined using this protocol, 2 products were found to have mixed with some beef or completely adulterated by beef.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第9期85-90,共6页
China Animal Husbandry & Veterinary Medicine
基金
“十一五”国家科技支撑计划项目(2007BAD52B05)
CAAS-ILRI联合研究项目(2008-CI-03)
中国农业科学院一级岗位杰出人才基金项目
关键词
七重PCR
羊肉产品
动物源性成分
种属鉴别
sepenary multiplex PCR
sheep and goat meal product
animal origin
species identification