摘要
目的获得Ｄ６Ｓ３６６、Ｄ６Ｓ４７７、Ｄ１２Ｓ３７５、Ｄ１２Ｓ３９１和ＰＬＡ２Ａ基因座在中国成都汉族群体中基因型及等位基因频率数据，初步探讨其在遗传学研究及法医学应用中的意义。方法随机抽取１０８名成都地区汉族群体无血缘关系个体的静脉血，ＥＤＴＡ抗凝，酚／氯仿提取ＤＮＡ。应用ＰＣＲ技术，扩增上述５个短串联重复序列基因座，聚丙烯酰胺凝胶垂直板电泳分型。结果中国成都汉族群体Ｄ６Ｓ３６６基因座发现７个等位基因，Ｄ６Ｓ４７７基因座发现９个等位基因，Ｄ１２Ｓ３７５基因座发现５个等位基因，Ｄ１２Ｓ３９１基因座发现９个等位基因，ＰＬＡ２Ａ基因座发现７个等位基因；５个基因座的基因型分布均符合Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡（Ｐ＞０．０５）。各基因座的杂合度分别为：０．６９、０．７８、０．８１、０．６８和０．７９，非父排除概率分别为：０．３６２１、０．６００４、０．４５８１、０．７０１４和０．５５８９，个人识别机率分别为：０．７９、０．９３、０．８６、０．９６和０．９１。结论５个基因座在中国成都汉族群体中具有较高的非父排除率与个人识别机率，在法医学应用和群体遗传学研究中有较高的价值。
Objective To get preliminary genotype and allele frequency distributions of D6S366,D6S477,D12S375,D12S391 and PLA2A loci in Chinese Han population in Chengdu area and to validate more short tandam repeat(STR) systems for forensic application.Methods The polymerase chain reaction(PCR) was used. Five STR systems(D6S366,D6S477,D12S375,D12S391 and PLA2A) were amplified on DNA samples, which were extracted from 108 unrelated individuals' EDTA blood by Phenol/Chloroform method. The PCR products were analyzed by PAG vertical electrophoresis.Results Seven alleles were found at D6S366 locus, nine alleles at D6S477 locus, five alleles at D12S375 locus, nine alleles at D12S391 locus and seven alleles at PLA2A locus. No deviations from Hardy Weinberg equilibrium were observed. The heterozygosities observed were 0.69,0.78,0.81,0.68 and 0.79 for D6S366,D6S477,D12S375,D12S391 and PLA2A respectively. The chances of exclusion were 0.3621,0.6004,0.4581,0.7014 and 0.5589 and the discriminating powers were 0.79, 0.93, 0.86,0.96 and 0.91.Conclusion All of the five loci in this study were useful markers for individual identification and paternity test and for genetics purposes.
出处
《中华医学遗传学杂志》
EI
CAS
CSCD
北大核心
1999年第2期79-80,共2页
Chinese Journal of Medical Genetics
关键词
短串联重复序列
PCR
遗传多态性
成都地区
汉族
Short tandem repeat Polymerase chain reaction Electrophoresis Polymorphism(Genetics)
