摘要
硫化物是沿海水域中常见的有害物质,对于多数生物来讲,其在微摩尔级水平便可导致生物体中毒。硫醌氧化还原酶(SQR)是一种谷胱甘肽还原酶家族的膜结合黄素蛋白,为硫化物氧化解毒的一种关键酶。本研究以体外原核表达并复性的单环刺螠SQR为材料,采用His-pulldown技术和SDS-PAGE分析,从单环刺螠体壁细胞裂解液中筛选出两个可能与SQR结合的蛋白质,分子质量分别为40 ku和60 ku。通过毛细管液相色谱-离子肼质谱分析,初步判断其为细胞色素P450(cytochrome P450)和腺苷三磷酸结合盒转运蛋白(ATP-binding cassette transporter),并初步探讨了其可能的功能。
Sulfide is toxic to most aerobic organisms, even fetal at a level of μmol/L to some organisms. Sulfide：quinone oxidoreduetase （SQR）, a membrane-bound flavoprotein of glutathione reductase family, is a key enzyme for sulfide oxidation detoxification. In this study, Urechis unicinctus SQR was expressed in Escherichia coli as inclusion bodies, and obtained active proteins by dilution refolding. Two probable SQR-binding proteins, which were of 40 and 60 ku respectively, were screened from the cell lysis solution of U. unicinctus body wall by His-pulldown technology and SDS-PAGE analysis, and preliminary determined as cytochrome P450 （cytochrome P450） and adenosine triphosphate-binding cassette transporter （ATP-binding cassette transporter） by mass spectrometer analysis. Probable functions of the two proteins were discussed.
出处
《海洋科学》
CAS
CSCD
北大核心
2010年第8期60-64,共5页
Marine Sciences
基金
国家自然基金面上项目资助(40776074)
