摘要
目的:建立HPLC同时测定血必净注射液中尿苷、没食子酸、鸟苷、丹参素钠、芍药苷、原儿茶醛、阿魏酸、红花黄色素A、洋川芎内酯I、洋川芎内酯H和丹酚酸B的方法。方法:采用Aglient Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm);流动相0.5%乙酸水(A),甲醇-乙腈-乙酸(40∶60∶0.5)(B)梯度洗脱;体积流量1 mL.min-1;检测波长280 nm;柱温35℃;自动进样20μL。结果:11种成分的线性关系良好,精密度、重复性、稳定性的RSD都低于3%,加样回收率为95.02%～104.94%。结论:本方法简便、快速、准确,能有效控制血必净注射液的质量。
Objective： To establish a HPLC method for the determination of 11 essential compounds in Xuebijing injection.Method： Chromatogaphic analysis was performed on Aglient Zorbax SB-C18（4.6 mm × 250 mm,5 μm）.The mobile phase 0.5% acetic acid and methanol-acetonitrile-acetic acid（40∶60∶0.5）.The flow rate was 1.0 mL·min^-1 with the detection wavelength at 280 nm;column temperature at 35 ℃,and automatic sampling volume of 20 μL.Result： All the 11 essential compounds showed good linearity（r=0.998 2-0.999 9）in the range of the tests concentration.The RSD of the precision,reproducibility and stability tests were less than 3%;the average recoveries of the method were in the range of 95.02%-104.94%.Conclusion： The method is simple,rapid and accurate,so it is proposed for the quality control of compounds of Xuebijing injection.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第18期2395-2398,共4页
China Journal of Chinese Materia Medica
基金
国家"十一五"科技支撑计划项目(2006BAI08B04-01)
