摘要
本文报道用卡介苗(BCG)和内毒素(LPS)诱导兎产生瘤肿坏死因子(TNF),获得含TNF血清(TNS)。TNS序顺通过DEAE-sephadex、SephadexG-200、Sephadex G-75和con-A-Agarose柱层析纯化,可获得单一电泳纯的TNF(SDS-PAGE和IEF),其分子量为67kD,等电点为pH5.2。抗肿瘤效应的结果表明,人胃癌细胞株:FGC-85和MGC-803对TNS/TNF敏感,SGC-7901不敏感;TNS/TNF可使小鼠移植性实体瘤(S_(180)、S_(37)、L_(Ⅱ)、U_(14)和黑色素瘤)发生出血性坏死,其中S_(180)对TNF最敏感。
Rabbit Tumor Necrosis Factor (TNF) was induced with BCG and LPS. TNF purified from the TNF containing serum (TNS) by sequential DEAE-Sephadex. Sephadex G-200, Sephadex G-75 and Gon-A-Agarose chromatography showed a single protein band on SDS-PAGE and IEF. The molecular weight of TNF was 67 kD, and its isoelectric point was pH5.2. The antitumor effect of TNS/TNF showed that in vitro human gastric cancer cell lines FGC-85 and MGC-803 were sensitive to TNF, and SGC-7901 was not;TNS/TNF could cause the hemorrhagic necrosis of transplanted murine tumors (S 180, S 37, LⅡ, U 14 and melanoma) in vivo. S 180 was among the most sensitive to TNF.
关键词
TNF
制备
纯化
抗肿瘤活性
Tumor Necrosis Factor (TNF), Antitumor activity, Human gastric ca cell lines, Transplanted murine tumors
