摘要
目的探讨VEGF-C及VEGF-D对人淋巴管内皮细胞增殖、促进淋巴管新生的机制。方法体外培养人淋巴管内皮细胞,用重组人VEGF-C、VEGF-D及VEGF-C+VEGF-D分别刺激后,计算细胞增殖率,比较各组细胞增殖情况。结果体外培养的淋巴管内皮细胞在加入重组人VEGF-C、VEGF-D试剂后细胞均增殖活跃。VEGF-D组增殖率为(54.2±4.35)%,VEGF-C组增殖率为(62.5±5.67)%,VEGF-C+VEGF-D组增殖率为(74.3±5.08)%,与对照组差异有显著性(P<0.05)。结论重组人VEGF-C与VEGF-D能刺激体外培养人淋巴管内皮细胞增殖。
Purpose To investigate the effects of VEGF-C and VEGF-D on proliferation of human lymphatic endothelial cells and explore mechanism of lymphangiogenesis induced by VEGF-C and VEGF-D.Methods The human lymphatic endothelial cells were cultured in vitro.Cells had been purified at the third passage.After stimulation with recombinant human VEGF-C（rhVEGF-C）,rhVEGF-D,rhVEGF-C＋rhVEGF-D,the proliferation of cells was compared in each group at last.Results The human lymphatic endothelial cells cultured in vitro has significantly proliferated after adding reagents.The proliferation rate of VEGF-D group was（54.2±4.35）%,the proliferation rate of VEGF-C group was（62.5±5.67）% and in VEGF-C＋VEGF-D group it was（74.3±5.08）%.They all had significant difference to the control group（P0.05）.Conclusion The supernatant of human recombinant VEGF-C and human recombinant VEGF-D cell cultured promote proliferation of lymphatic endothelial cells.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2010年第4期463-466,共4页
Chinese Journal of Clinical and Experimental Pathology
