摘要
目的：研究脂联素基因T45G多态性与广东省一般人群非酒精性脂肪肝病（NAFLD）发病易感性的关系。方法：对正常对照组人群507例随访4年，基线和终点均进行问卷调查、体检、血生化和B超检查，终点时人群分为NAFLD组（185例）与正常对照纽（322例），采用聚合酶链反应限制性片段长度法（PCR—RFLP）检测基因多态性，分析2组间基因型和等位基因的频率分布，用多因素Logistic回归分析新发脂肪肝的危险因素。结果：NAFLD组与正常对照组基因型（TT：47．57％US50．93％，TG：45．40％vs42．24％，GG：7．03％US6．83％）和等位基因（T：70．27％掷72．05％，G；29．73％US27．95％）差异无统计学意义（P〉0．05）。按体重指数（BMI）正常与否分层后，2组间3种基因型频率的分布差异也无统计学意义（P〉0．05）。结论：脂联素基因T45G多态性与NAFLD的发病易感性不相关。
Objective： To investigate the correlation between adiponectin gene T45G polymorphisms and susceptibility of non-alcoholic fatty liver disease（NAFLD） in Guangdong Province. Methods： 507 normal control cases out of general population in Guangdong Province were prospectively followed-up for 4 years. Questionnaires, physical examination, blood biochemical tests and abdominal uhrasonography were carried out for each subject on the baseline and endpoint. The participants were divided into 2 groups at the endpoint ： NAFLD （n = 185 ） and normal control（ n = 322）. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was applied to detect the single nucleotide polymorphisms （SNPs）. The genotype and allelic frequency distribution between 2 groups were analyzed. Multivariate regression logistic analysis was applied to identify the risk factors of incidental NAFLD. Results ： The adiponectin genotypes （TT：47.57% vs 50.93% , TG：45.40% vs 42.24% , GG：7.03% vs 6. 83% ）and allelic frequencies （T：70.27% vs 72.05%, G：29.73% vs 27.95% ） were not significantly different between NAFLD and normal control groups （ P 〉 0.05 ）. When the participants were divided into two subgroups according to their BMI abnormality,the adiponectin genotypes were also not significantly different. （P 〉 0.05 ）. Conclusion： The T45G polymorphisms of adiponectin gene were not shown to be associated with NAFLD susceptibility.
出处
《广州医学院学报》
2010年第2期1-5,共5页
Academic Journal of Guangzhou Medical College
基金
广州市卫生医药卫生科技重点项目（2008-ZDi-1）和（2009-ZDi-03）.
关键词
非酒精性脂肪性肝病
脂联素基因
多态性
易感性
non-alcoholic fatty liver disease
adiponeetin gene
polymorphism
susceptibility
