摘要
目的:分离主导管结扎后损伤下颌下腺组织中的干/祖细胞(Salivary Gland Progenitor,SGP),进行长期培养,对10～25代的染色体进行分析。方法:结扎SD大鼠下颌下腺主导管,获得损伤模型,机械及酶消化法体外分离培养腺体细胞,获得类上皮细胞集落,挑取集落后梯度稀释法纯化获得类上皮单克隆细胞-涎腺干/祖细胞,对长期培养的SGP行染色体常规及G-带核型分析。结果:SGP染色体数2n=42,其中常染色体20对,性染色体1对;SGP染色体数目、形态及结构未发现特异性异常改变。结论:长期传代培养的SGP二倍体性状不改变,遗传性状相对稳定。
Objective：To isolate salivary gland progenitor cells from damaged submandibular gland and analyze chromosome G-banding karyotype by long-term culture.Methods：Tissue trauma was performed by ligation of main duct of submandibular gland in Sprague-Dawlye（SD）rats.Cells were isolated by mechanical method and enzyme digestion.With limited dilution,cell line purified from epithelium-like colony was designated as salivary gland progenitor cells and G-banding karyotype was analyzed by long-term culture.Results：The chromosome number of SGP cell line was 2n=42with no abnormality in number,morphology and structure.Conclusion：After long time culture,the SGP cell line maintained its digenomous diploid without abnormality so as to prove their inheritancestability.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2010年第4期512-514,共3页
Journal of Oral Science Research
基金
贵州省科学技术厅科技攻关项目(2003)53号
贵州省优秀科技教育人才省长基金(黔省专合字(2008)113号
贵州省教育厅基金项目(2006)354号
关键词
涎腺干/祖细胞
染色体
核型分析
Salivary gland progenitor cells（SGP） Chromosome Karyotype
