摘要
采用UV-vis光谱和荧光光谱法研究双酚A(BPA)与牛血清白蛋白(BSA)的相互作用.结果显示,BPA的加入能使BSA肽链伸展,导致色氨酸,酪氨酸和苯丙氨酸等残基的芳杂环裸露,紫外光谱表现为明显的增色效应.在三个给定的温度(25℃,37℃和42℃)下,BPA对BSA的荧光猝灭方式均为静态猝灭,结合位点数(n)均为1,作用距离(r)均小于7 nm.BPA与BSA的相互作用是自发进行的,主要为疏水作用.同步荧光光谱表明,BPA对BSA的色氨酸残基微区的影响大于对酪氨酸残基微区的影响.酸度和离子强度的增加促进BPA与BSA的相互作用.另外,通过UV-vis光谱和高效液相色谱(HPLC)研究了活性炭催化微波照射下BPA的降解液对BSA的作用.结果表明,此法可有效地降解BPA,但其降解液仍然对BSA作用.而且即使经过较长时间(5.0 min)微波照射和多次稀释方式处理的BPA降解液仍然对BSA具有一定的作用.
The interaction between bisphenol A（BPA） and bovine serum albumin（BSA） was investigated by UV-vis and fluorescence spectroscopy.The results revealed that the addition of BPA caused the stretch of peptide chains and the exposure of aromatic heterocycle of tryptophan,tyrosine and phenylalanine etc residues and the obvious hyperchromic effect was observed in UV-vis spectrum.At three given temperatures（25 ℃,37 ℃ and 42 ℃）,the quenching modes between BPA and BSA were all static quenching.The number of the three binding sites（n） all equaled one and the binding distances（r） were less than 7 nm.The interaction of BPA with BSA was spontaneous and mainly hydrophobic.Synchronous fluorescence spectra indicated that the influence of BPA on the micro-zone of the tryptophan residues of BSA was greater than that on tyrosine residues.Also,the interaction of BPA with BSA was promoted by the increase of acidity and ionic strength.Furthermore,the degradation of BPA under microwave irradiation catalyzed by activated carbon was studied using UV-vis spectrum and HPLC.The results showed that it was an effective way to degrade BPA.However,the degraded solution of BPA still interacted with BSA even though the 5.0 min irradiation and dilution for several times were adopted to deal with BPA.
出处
《环境化学》
CAS
CSCD
北大核心
2010年第5期835-841,共7页
Environmental Chemistry
基金
辽宁省教育厅自然科学基金(L2010156)
