摘要
以豆秸粉为主要原料,通过单因素和正交试验确定金针菇(Flammulina velutipes)固态发酵生产木聚糖酶最佳培养条件,并对其粗酶的酶学性质进行了研究.结果表明,碳源为豆秸粉:玉米芯:麸皮=7:2:1,氮源为1.5%的黄豆粉,pH为5,接种量为干料的20%,装瓶量为6 g/100 mL三角瓶装,料水比为1:2.1,培养天数5 d,在此培养条件下得到的酶活力最高,产量可达52 768.91 U/g.粗酶液的最适反应温度为60℃,最适反应pH为6.酶的pH稳定性及热稳定性较好,80℃时相对酶活力仍保持在40.41%.
Soybean stem meal was used as main material to produce xylanase with Flammulina velutipes. Monofactorial and orthogonal experiments were performed to optimize the solid-phase fermentation conditions, and the enzymatic characteristics of crude enzyme liquid were discussed. The results showed that the optimal culture conditions for enzyme producing were： the mixture ratio of soybean stem meal and corn cob and wheat bran is 7/2/1, 1.5% soybean flour as nitrogen source, pH5, inoculum＇s volume 20% for 5 days, 6 g medium in 100 mL Erlenmeyer flask, the ratio of solid to water 1/2.1. The enzyme yielding is about 52768.91 U/g. The optimal enzyme reaction condition is under pH6 at 60℃. The enzyme thermal stability is better between pH3 - 8, and 40.41% enzyme activity will be remained at 80℃.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第3期472-477,共6页
Journal of Central China Normal University：Natural Sciences
基金
安徽省教育厅自然科学基金(KJ2009B168Z)
安徽科技学院生物学重点建设学科项目(AKXK2010 2-1)
关键词
木聚糖酶
金针菇
固态发酵
正交试验
xylanase
flammulina velutipes
solid state fermentation
orthogonal test
