摘要
目的:探讨血管内皮生长因子C(VEGF-C)的表达对胃食管交界癌淋巴管生成及患者预后的影响。方法:免疫组化法检测128例行根治术的胃食管交界癌组织中VEGF-C的表达,采用LeicaIM50系统采集图像,Image-pro○RPlus6.0软件计算平均光密度值(MOD);D2-40标记淋巴管并计算淋巴管密度(LVD)。结果:VEFG-C高、低表达组(MOD>0.18,≤0.18)的LVD值分别为16.90±5.96和13.60±5.58条,P=0.002,送检阳性淋巴结数分别为2.90±2.44和2.00±2.36枚,P=0.025;LVD高、低水平组(LVD>13条,≤13条)的阳性淋巴结送检数分别为3.00±2.34和1.90±2.43枚,P=0.010。单因素生存分析显示,高VEGF-C表达组及高LVD组的患者无瘤生存情况明显差于相应的低表达/水平组。多因素分析提示,VEGF-C高表达为独立于其他临床因素(TNM分期、有否化疗、手术切缘范围)的预后不良影响因素。结论:胃食管交界癌VEGF-C高表达与LVD正相关,是独立的预后不良影响因素。
OBJECTIVE：To clarify the impact of VEGF-C on lymphangiogenesis and clinical outcome in gastro-esophageal junction carcinoma.METHODS：VEGF-C expression was detected immunohistochemically in 128 specimens of gastro-esophageal junction carcinoma that underwent curative sugery.The mean optical density （MOD） was measured with the digital image analysis system （Leica IM50 image acquisition system,Image-pro○R Plus 6.0 software） to represent the expression level of VEGF-C.The lymphatic vessel was labeled with D2-40 antibody to calculate lymphatic vessel density （LVD）.RESULTS：In the high （MOD0.18） and low VEGF-C groups （MOD≤0.18）,the mean LVDs were 16.90±5.96 and 13.60±5.58 respectively （P=0.002）,the mean numbers of positive resected lymph nodes were 2.90±2.44 and 2.00±2.36 respectively （P=0.025）.In the high （LVD13） and low level of LVD groups （LVD≤13）,the mean numbers of positive resected lymph nodes were 3.00±2.34 and 1.90±2.43 respectively （P=0.010）.The univariate analysis showed the high expression levels of VEGF-C and LVD were all statistically associated with the poor disease-free survival.The multivariate analysis indicated that the high expression of VEGF-C was unfavorable prognostic factor independence of other clinical factors,including TNM stage,chemotherapy,and surgical margin for gastro-esophageal junction carcinoma.CONCLUSION：The high expression of VEGF-C correlates with high LVD and is independent unfavorable prognostic factor for gastro-esophageal junction carcinoma.
出处
《中华肿瘤防治杂志》
CAS
2010年第16期1285-1288,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
广东省科技厅计划项目(2005B30301010)
关键词
胃食管交界癌
血管内皮生长因子C
淋巴管生成
预后
gastro-esophageal junction carcinoma
vascular endothelial growth factor C
lymphangiogenesis
prognosis
