摘要
目的探讨深圳市宝安区乙型肝炎患者血清中乙肝病毒(HBV)基因型的分布情况,了解各基因型与HBV-DNA水平、HBeAg、肝损程度、抗病毒疗效、疾病进展和预后关系。方法采用S基因片段测序的基因分型法,通过上下游引物经PCR反应体系和操作程序扩增S基因片段,经1.5%琼脂糖凝胶电泳,用3730双向测序技术,将130例HBV-DNA阳性患者测序结果在NCBI上进行HBV基因分型,得出基因型。结果宝安区HBV基因型只有B型和C型,无其他型和混合型,B型占55.74%,C型占44.26%,无性别和年龄分布差异(P>0.05)。B型与C型HBV-DNA水平无显著性差异(P>0.05),B基因型HBeAg阳性率较C基因型高,C型肝损程度比B型重,进展为肝硬化、肝细胞性肝癌的几率高,两者相比有显著性差异(P<0.01)。结论研究病例HBV基因型以B型为主,C型次之。C基因型患者病情重,进展快,预后差,值得引起临床医生重视。
Aim To explore the distribtition of serum hepatitis B virus genotype in patiens with hepatitis B in Baoan District of Shenzhen. Methods S-gene segments were amplified through upsteam and downsteam primers by PCR reaction system with 1.5% agarose gel electrophoresis ,the DNA fragments of 130 HBV-DNA-positive patients were sequened on 3730XL DNA Analyzers. The genotypie assay was completed. Results There were only two genotypes （type B and type C） in HBV-positive patients in Baoan District. Their percentage of tpye B and type C accounted for 55.74%,44.26% respectively; The distribution had no differences between sex and age（P〉0.05）.. The positive rate of genotype B was higher than that of genotype C. While level damage in genotype C was severer with higher probability of development to cirrhosis and hepatoeellular carcinoma, showing significant diffeences （P〈0.01）. Conclusion In Baoan District genotype B is predominant followed by genotype C. HBV-positive patients of genotype C develop rapidly progress with poor proghosis.
出处
《中国热带医学》
CAS
2010年第11期1319-1321,共3页
China Tropical Medicine
基金
深圳市宝安区2008年科研立项课题(2008180)
