摘要
目的建立双瓜糖安胶囊中黄芪甲苷的HPLC-ELSD测定方法。方法采用高效液相色谱-蒸发光散射检测法(HPLC-ELSD),色谱柱为Agilent-C18(4.6mm×150mm,5μm),流动相乙腈-水(30∶70),柱温35℃,流速1.6L/min。结果测定了四批双瓜糖安胶囊中黄芪甲苷的含量,建立了该制剂中黄芪甲苷的含量测定方法。结论该方法简便、快速、灵敏、准确、重复性好,专属性强,可作为双瓜糖安胶囊的质量控制方法。
Objective To build up a method for determination of astragalosideⅣ in Shuangguatangan Capsules by HPLC-ELSD.Methods HPLC-ELSD was used in the quantitative analysis with Agilent-C18 chromatography column（4.6 mm × 150 mm,5 μm） and acetonitrile-water（30：70） as amobile phase.The column temperature was set to 35℃.The flow rate of mobile phase was 1.6 L/min.Results Determining astragaloside Ⅳ in four groups of Shuangguatangan Capsules,then develop a method for determination of astragalosideⅣ in sample.Conclusion This method is simple、rapid、reproducible and can be regarded as the quality control method of Shuangguatangan Capsules.
出处
《中国现代药物应用》
2010年第18期31-33,共3页
Chinese Journal of Modern Drug Application
