摘要
在温度（２０±１）℃和光照１２Ｌ ： １２Ｄ的条件下，用萨氏培养液对安徽虫瘟霉（Ｚｏｏｐｈｏｒａ ａｎ－ ｈｕｉｅｎｓｉｓ）进行液体振荡培养（１００ ｒ／ｍｉｎ），考查不同接种量和液培时间对菌丝生物量的影响。结果表明， 将含干菌丝１８． ６ｍｇ／ｍＬ的母液按１％～１５％（Ｖ／Ｖ）的比例接种后培养４８～７２ｈ，９％和１２％接种量处 理能产生２０ ｍｇ／ｍＬ 以上的菌丝生物量，通过拟合逻辑斯蒂生长模型，发现该菌在上述液培条件下的最 大菌丝生物量为２５ ｍｇ／ｍＬ左右。
The entomophthoralean fungus, Zoophthora anhuiensis,was investigated in shaking (100 r/min) culture of Sabouraud dextrose broth at the regime of 20 and 12L : 12D to evaluate the effect of initial inocula on mycelial biomass. Seed culture containing 18. 6 mg dry mycelia per milliliter was used to initiate 50 mL liquid culture at the proportions (V/V) of 1%/~15%. It was found that after incubation of 48~72 h the cultures initiated 9% and 12% seed culture yielded a mycelial biomass of >20 mg/mL. The firting of the data to Logistic growth medel showed that Z. anhuiensis may produce a maximum biomass of ca. 25mg/mL at the above regime.
基金
国家杰出青年科学基金!3925004
国家自然科学基金!39770016
关键词
安徽虫瘟霉
液体培养
初始接种量
菌丝生物量
Zoophthora anhuiensis, liquid culture , initial inoculum, mycelial biomass , growth model
