摘要
目的:研究人肝内胆管癌组织中Ki67抗原和P16蛋白的表达及其意义。方法:应用免疫组化S-P法对112例人肝内胆管癌组织石蜡切片行Ki67抗原和P16蛋白染色分析。结果:①肝内胆管癌Ki67指数(35.9±20.0)显著高于肝内胆管结石症和正常肝内胆管(P<0.001),并随癌分化程度的降低而增高(P<0.001);淋巴结转移组Ki67指数高于无淋巴结转移组(P<0.01)。②P16蛋白表达阳性率在肝胆管癌组织、胆石症增生胆管上皮、正常肝内胆管上皮各组间分别为55.4%、78%、93.3%,呈递减趋势(P<0.05),并随癌分化程度降低而减低(P<0.05)。③Ki67抗原与P16蛋白表达呈负相关,随Ki67-LI值升高P16蛋白表达减低(P<0.01)。结论:Ki67抗原作为细胞增殖指标其过量表达与肝内胆管癌恶性生物学行为,特别是淋巴结转移密切相关,可能应用临床判断肿瘤转移危险性和估计预后。P16蛋白缺失与肝内胆管癌的发生有关。
Objective：Investigate the expression and biologic significance of Ki67 antigen and p16 protein in human intrahepatic cholangiocarcinoma.Methods：Immunohistochemistry was used to detected the Ki67 antigen and p16 protein in 112 cases of intrahepatic cholangiocarcinoma tissues.Results：1、The Ki67-LI of cholangiocarcinoma（35.9±20.0） was significantly higher than that of hepatolithiasis and normal hepatic bile duct （P〈0.001）;the espression of Ki67 antigen was significantly related to tumor differentiation and the metastases to local lymph nodes（P〈0.01）.2、The expression level of p16 protein showed as follow：cholangiocarcinoma〈hepatolithiasis〈normal tissues（P〈0.05）;compared with well-differentiated cancer,the positive rate of p16 protein was lower in poor-differentiated cancer （P〈0.05）.3、Expression of p16 protein was in negative correlation with the expression of Ki67 antigen.Conclusion：Overexpression of Ki67 antigen was closely related to the biological behaviours of cholangiocarcinoma,it can be used as an objective marker for predicting progonosis of cholangiocarcinoma and to foretell the high risk of metastasis;the deletion of p16 protein expression is related to the carcinogensis of cholangiocarcinoma.
出处
《医学信息（中旬刊）》
2010年第10期2739-2740,共2页
Medical Information Operations Sciences Fascicule
